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中国农机化学报

中国农机化学报 ›› 2021, Vol. 42 ›› Issue (1): 1-8.DOI: 10.13733/j.jcam.issn.2095-5553.2021.01.001

• 中国农机化学报 •    下一篇

粮食安全与农业机械化的逻辑关系及其政策启示——基于三种主要粮食种植成本收益的中美比较

姚春生;侯方安;陈海燕;   

  1. 农业农村部农业机械试验鉴定总站农业农村部农业机械化技术开发推广总站;北京联合大学人力资源管理研究所;
  • 收稿日期:2016-12-05 修回日期:2016-12-20 接受日期:2017-01-02 出版日期:2021-01-15 发布日期:2021-01-15
  • 通讯作者: 沈春修
  • 作者简介:沈春修(1979—),男,湖南溆浦人,讲师,博士,主要从事水稻抗逆性研究。E-mail:shenchunxiu@126.com

CRISPR/Cas9 editing and expression analysis of LOC_Os10g05490 in rice under cold stress

SHEN Chunxiu   

  1. College of Life Sciences, Resources and Environment, Yichun University, Yichun 336000, China
  • Received:2016-12-05 Revised:2016-12-20 Accepted:2017-01-02 Online:2021-01-15 Published:2021-01-15
  • Contact: Shen Chunxiu
  • About author:Shen Chunxiu,(1979—),E-mail:shenchunxiu@126.com

摘要: 我国2016—2018年稻谷、小麦、玉米种植连续出现亏损。如何增加粮食种植纯收益,提高农民种粮积极性,保障粮食安全?在新冠疫情全球蔓延的特殊时期,粮食安全问题又在我国引起关注。通过比较中美两国三种主要粮食作物种植成本与收益,认为人工成本的攀升是我国粮食种植总成本不断升高的主要原因,提高种植效益的实现路径是通过发展农业机械化来减少人工投入,但我国已开始进入农业机械过度投资的结构性困境。为此,提出如下政策建议:采取更积极措施解决劳动密集的瓶颈环节机械化技术问题;支持发展农机作业社会化服务;推进土地流转实现规模经营;加强有利于农机作业的基础设施建设。

关键词: 粮食安全, 农业机械化, 粮食作物, 成本, 收益

Abstract: To explore the expression and cold tolerance function of LOC_Os10g05490 in rice under cold stress, semi-quantitative analysis was performed for Dongxiang wild rice and 93-11. The results showed that expression level of LOC_Os10g05490 in 93-11 was the same before and after cold stress, but it was down regulated in Dongxiang wild rice after cold stress. This was consistent with RNA-seq data and further study indicated that this gene might be associated with cold tolerance in Dongxiang wild rice. Plant expression vectors with CRISPR/Cas9 genome editing technique were subsequently constructed to target the LOC_Os10g05490 locus, and were transformed into the japonica rice variety TP309. A total of 26 transgenic positive plants were obtained. This study may lay the foundation for subsequent screening of mutant plants and identification of genes associated with cold tolerance in rice.

Key words: cold stress, RNA-seq, Dongxiang wild rice, CRISPR/Cas9 genome editing technique

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