关键词: 啶虫脒, 农药残留, 电化学检测, 适体传感器

Abstract: In order to know well the pollution of pesticides on agricultural environment and crops, it is an important demand to study the rapid and accurate pesticide residue detection methods at present. In this paper, a new detection method based on electrochemical biosensor technology was proposed for the detection of acetamiprid residues in soil. An aptamer sensor suitable for the detection of acetamiprid residues in soil was developed. A soil pesticide residue detection device based on electrochemical sensing technology was designed. It had the functions of field soil collection, pretreatment, and onsite detection for acetamiprid residue content in soil. Soil sampling mechanisms, mixing mechanisms, and pesticide residue detection units were designed. The key design parameters of each mechanism were simulated and calculated. According to the analysis results, the devices of the soil sampling pretreatment device were selected, and the control scheme of the electrical control system was determined. Experimental verification was conducted on the detection performance of the developed device. The results of soil sample collection experiments showed that when the depth of the soil layer was 0-5cm, the relative standard deviation of soil sample quality was less than 2%, and the columnar soil sample would not adhere to the surface of the alloy drill bit. Compared with commercial electrochemical workstations, the average error of the measured concentration of acetamiprid was only 0.7%, verifying the accuracy of the developed device detection. This study provides new detection methods and technical support for rapid detection of pesticide residues.

Key words: acetamiprid, pesticide residues, electrochemical detection, aptamer sensors